Antimicrobial activity of natural products from the flora of Northern Ontario, Canada
Type of Spiritual Experience
A description of the experience
Pharm Biol. 2015 Jun;53(6):800-6. doi: 10.3109/13880209.2014.942867. Epub 2015 Feb 20.
Antimicrobial activity of natural products from the flora of Northern Ontario, Canada.
Vandal J1, Abou-Zaid MM, Ferroni G, Leduc LG.
- 1Department of Biology, Laurentian University , Sudbury, Ontario , Canada .
The number of multidrug resistant (MDR) microorganisms is increasing and the antimicrobial resistance expressed by these pathogens is generating a rising global health crisis. In fact, there are only a few antimicrobial agents left that can be used against MDR bacteria and fungi.
In this study, the antimicrobial activities of selected natural products from the flora of Northern Ontario against selected microorganisms are reported.
MATERIALS AND METHODS:
Plants were collected from Sault Ste. Marie, Ontario, Canada, and ethanol extracts were prepared using EtOH:H2O (1:1, v/v). Fungal cultures used in this study were Candida albicans ATCC 10231 and Schizosaccharomyces octosporus. Bacterial cultures employed included Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Mycobacterium phlei ATCC 11758, and Streptococcus lactis ATCC 19435. The microplate resazurin assay was used to screen for antimicrobial activity.
Extracts of four plant species Chimaphila umbellata L. (Pyrolaceae), Betula papyrifera Marshall (Betulaceae), Rhus typhina L. (Anacardiaceae), and Fraxinus pennsylvanica Marshall (Oleaceae), and six compounds (gallic acid, ethyl gallate, caffeic acid, sinapic acid, gentisic acid, and chlorogenic acid) demonstrated antibacterial or antifungal activities with MICs ranging from 62.5 to 1000 µg/mL, respectively, for a chemical fraction of an extract from Betula papyrifera against the bacterium S. aureus.
DISCUSSION AND CONCLUSION:
The present study has shown that certain plant extracts and select fractions and standard chemical compounds exhibit antimicrobial effects.
- Prince's Pine, Chimaphila umbellate,
- White Birch, Betula papyrifera,
- Staghorn Sumac, Rhus typhina, and
- Green Ash, Fraxinus pennsylvanica
were the principal extracts exhibiting notable antibacterial and/or antifungal activities; while gallic acid, ethyl gallate, and caffeic acid demonstrated antibacterial activities and sinapic acid, gentisic acid, and chlorogenic acid demonstrated antifungal activities.
Betula; C. albicans; E. coli; Fraxinus; P. aeruginosa; S. aureus; chimaphila; phenolics
The source of the experiencePubMed
Concepts, symbols and science items
Activities and commonsteps
Fungal infection treatments
Pseudomonas aeruginosa infection