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Food dyes and genotoxicity - Food manufacturing companies

Identifier

014505

Type of Spiritual Experience

None
Hallucination

Number of hallucinations: 1

Background

Hormones secreted from the pituitary gland help control: growth, blood pressure, certain functions of the sex organs, thyroid glands and metabolism as well as some aspects of pregnancy, childbirth, nursing, water/salt concentration and the kidneys, temperature regulation and pain relief.

A description of the experience

Food Chem Toxicol. 2012 Oct;50(10):3447-51. doi: 10.1016/j.fct.2012.07.042. Epub 2012 Jul 28.

Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells.

Chequer FM1, Venâncio VP, Bianchi ML, Antunes LM.

Erythrosine (ErB) is a xanthene and an US Food and Drug Administration approved dye used in foods, drugs and cosmetics. Although its utilization is permitted, ErB is described as inhibitor of enzymes and protein-protein interactions and is toxic to pituitary and spermatogenesis processes.

However, the genotoxicity and mutagenicity of ErB is inconclusive in the literature. This study aimed to analyze the genotoxicity of this dye using the alkaline comet assay and is the first investigation to evaluate ErB mutagenicity using the cytokinesis block micronucleus cytome (CBMN-Cyt) assay in HepG2 cells. These cells were chosen because they produce phase I and phase II enzymes that can mimic in vivo metabolism. The cells were treated with seven concentrations (0.1-70.0 μg mL(-1)) of ErB, and the results showed genotoxicity at the two highest concentrations and mutagenicity at six concentrations.

Furthermore, as micronuclei result from clastogenic and aneugenic processes, while comet assay is often considered more sensitive and detects DNA single strain breaks, we suggest that an aneugenic is responsible for the observed damage.

Although ErB is approved for use in the food, cosmetic and pharmaceutical industries, it must be used carefully because it damages the DNA structure.

Copyright © 2012 Elsevier Ltd. All rights reserved.

PMID: 22847138

The source of the experience

PubMed

Concepts, symbols and science items

Concepts

Symbols

Science Items

Food additives

Activities and commonsteps

Commonsteps

References