Fabin, a novel calcyon-like and glucanase-like protein with mitogenic, antifungal and translation-inhibitory activities from broad beans
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Biol Chem. 2003 May;384(5):811-5. Fabin, a novel calcyon-like and glucanase-like protein with mitogenic, antifungal and translation-inhibitory activities from broad beans. Ng TB1, Ye XY.
A protein with an N-terminal sequence displaying similarities to N-terminal sequences of human calcyon and barley endo-1,4-glucanase, and to C-terminal sequences of human translation initiation factor 4 gamma and yeast superkiller viralicidic activity, was isolated from the broad bean Vicia faba.
The protein, termed fabin, has a molecular mass of 34 kDa in SDS-polyacrylamide gel electrophoresis.
Antifungal activity of the protein was observed against several fungal species including Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum and Mycosphaerella arachidicola.
Fabin inhibits HIV-1 reverse transcriptase with an IC50 of 34 microM and translation in a rabbit reticulocyte lysate with an IC50 of 2.4 microM.
At a concentration of about 1.5 microM fabin is able to elicit a 9-fold increase in the mitogenic response of murine splenocytes.