Biofactors. 2014 Nov-Dec;40(6):611-23. doi: 10.1002/biof.1173. Epub 2014 Oct 30.

Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: potential impact on colonic health.

Brown EM1, Nitecki S, Pereira-Caro G, McDougall GJ, Stewart D, Rowland I, Crozier A, Gill CI.

• 1Northern Ireland Centre for Food and Health, Centre for Molecular Biosciences, University of Ulster, Coleraine, Northern Ireland, UK.

Abstract

The composition of polyphenols in ileal fluid samples obtained from an ileostomy subject after lingonberry intake was compared with lingonberry extracts obtained after simulated in vitro digestion (IVDL) and subsequent faecal fermentation (IVFL). HPLC-PDA-MS/MS analysis confirmed similar patterns of lingonberry (poly)phenolic metabolism after the in vivo and in vitro digestion, with reduced recovery of anthocyanins and a similar pattern of recovery for proanthocyanidins observed for both methods of digestion. On the other hand, the IVFL sample contained none of the original (poly)phenolic components but was enriched in simple aromatic components. Digested and fermented extracts exhibited significant (P < 0.05) anti-genotoxic (Comet assay), anti-mutagenic (Mutation Frequency assay), and anti-invasive (Matrigel Invasion assay) effects in human cell culture models of colorectal cancer at physiologically-relevant doses (0-50 μg/mL gallic acid equivalents). The ileal fluid induced significant anti-genotoxic activity (P < 0.05), but at a higher concentration (200 μg/mL gallic acid equivalents) than the IVDL. Despite extensive structural modification following digestion and fermentation, lingonberry extracts retained their bioactivity in vitro. This reinforces the need for studies to consider the impact of digestion when investigating bioactivity of dietary phytochemicals.

© 2014 International Union of Biochemistry and Molecular Biology.

KEYWORDS:

(poly)phenol; anti-genotoxic; colorectal cancer; digestion; ileostomy; lingonberry

PMID:

25359330