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Inhibitory effects of O-methylated isoflavone glycitein on human breast cancer SKBR-3 cells

Identifier

017891

Type of Spiritual Experience

Background

Balance is key...........................

Another example

….. phytoestrogen induces significant inhibitory effects on the growth of breast and ovarian cancers through different signaling pathways. …. Phytoestrogen potentially modulates the signaling molecules via:

(1) blocking the nuclear and membrane estrogen receptors (ER),
(2) interfering with the growth factor receptor,
(3) inhibiting the G protein-coupled receptor in ER-deficient cells,
(4) activating apoptosis and nullifying anti-apoptotic signals. 

PMID:  21617769

A description of the experience

Int J Clin Exp Pathol. 2015 Jul 1;8(7):7809-17. eCollection 2015.

Inhibitory effects of O-methylated isoflavone glycitein on human breast cancer SKBR-3 cells.

Zhang B1, Su JP2, Bai Y1, Li J1, Liu YH1.

  • 1Department of Surgical Oncology, Cangzhou Central Hospital Cangzhou, China.
  • 2Department of Endocrinology, Cangzhou People's Hospital Cangzhou, China.

Abstract

Glycitein is an O-methylated isoflavone which accounts for 5-10% of the total isoflavones in soy food products. Cell proliferation studies on the dietary phytoestrogen, glycitein against human breast carcinoma SKBR-3 cells showed that glycitein exhibits biphasic regulation on SKBR-3 cells. At concentrations of less than 10 mg/mL, cells respond to glycitein by increasing cell growth and de novo DNA synthesis whereas the addition of glycitein at concentrations greater than 30 mg/mL significantly inhibited cell growth and DNA synthesis in a dose-dependent manner. Cells treated with 60 mg/mL of glycitein did not regain normal growth after treatment was stopped. Glycitein was found to be cytostatic at low concentrations and cytotoxic at higher concentrations. Treatment with 100 mg/mL of glycitein severely altered the cell morphology. Collective results showed that glycitein damaged the cell membranes by increasing membrane permeability and suggested possible mechanisms of the action of dietary phytoestrogens on human breast carcinoma SKBR-3 cells.

KEYWORDS:

Glycitein; SKBR-3 cells; breast carcinoma

PMID:  26339345

The source of the experience

PubMed

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