In vitro and in vivo antileishmania activity of sesquiterpene lactone-rich dichloromethane fraction obtained from Tanacetum parthenium (L.) Schultz-Bip
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Exp Parasitol. 2014 Aug;143:18-23. doi: 10.1016/j.exppara.2014.04.014. Epub 2014 May 5.
In vitro and in vivo antileishmania activity of sesquiterpene lactone-rich dichloromethane fraction obtained from Tanacetum parthenium (L.) Schultz-Bip.
Rabito MF1, Britta EA1, Pelegrini BL1, Scariot DB1, Almeida MB2, Nixdorf SL2, Nakamura CV1, Ferreira IC3.
- 1Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Maringá, PR 87020-900, Brazil.
- 2Programa de Pós-graduação em Química, Universidade Estadual de Londrina, Londrina, PR 86057-970, Brazil.
- 3Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Maringá, PR 87020-900, Brazil. Electronic address: firstname.lastname@example.org.
The discovery of new treatments for neglected diseases, including leishmaniasis, is a substantial challenge for scientific research. Plant extracts have shown potential in the selective treatment of tropical diseases.
The present study evaluated the in vitro and in vivo antileishmania effects of a sesquiterpene lactone-rich dichloromethane fraction (DF) obtained from the aerial parts of Tanacetum parthenium (L.) Schultz-Bip. In vitro studies of the DF indicated an IC50 of 2.40±0.76 μg mL(-1) against the promastigote form and 1.76±0.25 μg mL(-1) against the axenic amastigote form of Leishmania amazonensis. In vivo intramuscular treatment with DF decreased the growth and size of footpad lesions in mice. The DF also significantly decreased the parasite population compared with animals that were treated with the reference drug. Plasma malondialdehyde levels were increased slightly by the DF, attributable to its parthenolide-rich composition that causes cellular apoptosis, compared with the control group, demonstrating treatment efficacy without toxicity or genotoxicity. Because the isolation and purification of plant compounds are costly and time-consuming and generate low yields, extract fractions, such as the DF studied herein, represent a promising alternative for the treatment of leishmaniasis.
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Leishmania amazonensis; Leishmaniasis; Phytochemical