J Agric Food Chem. 2013 Apr 24;61(16):3884-90. doi: 10.1021/jf400390e. Epub 2013 Apr 12.

Eudesmanolides from Wedelia trilobata (L.) Hitchc. as potential inducers of plant systemic acquired resistance.

Li Y1, Hao X, Li S, He H, Yan X, Chen Y, Dong J, Zhang Z, Li S.

Author information

• 1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650201, Yunnan, China.

Abstract

Ten eudesmanolides (1-10) including five new ones (1-4 and 6) were isolated from the whole plant of Wedelia trilobata (L.) Hitchc., a notoriously invasive weed in South China. As main constituents of W. trilobata, eight of these eudesmanolides were tested for their antitobacco mosaic virus (TMV) activities by the conventional half-leaf and leaf-disk method along with Western blot analysis. All of the tested compounds, at 10 μg/mL, showed strong antiviral activities in the pretreated tobacco plants with inhibition rates ranging from 46.7% to 76.5%, significantly higher than that of the positive control, ningnanmycin (13.5%). Their potential of inducing systemic acquired resistance (SAR) was also evaluated, and compounds 1 and 8 showed excellent induction activities. Furthermore, it was found that different concentrations of compound 1 promoted phenylalanine ammonia-lyase (PAL) activity in tobacco plants. To our knowledge, this is the first report that eudesmanolides could induce resistance in tobacco plants against the viral pathogen TMV.

PMID:

23537063