Coffee as a laxative
Type of Spiritual Experience
A description of the experience
New biological properties of coffee melanoidins. Argirova MD, Stefanova ID, Krustev AD.
Department of Chemistry and Biochemistry, Medical University, 120 Buxton Brothers Str., 4004 Plovdiv, Bulgaria.
Water-soluble melanoidins isolated from roasted coffee induced ex vivo changes in the bioelectric and contractile activity of rat circular gastric smooth muscle tissues.
They provoked a depolarization of smooth muscle cellular membranes and an increase in Ca²-influx as evidenced by the increase in the frequency and amplitude of Ca²-generated spike potentials. In the presence of 1 × 10 mol L¹ acetylcholine and 1 × 10 mol L¹ arecoline the melanoidin-evoked contraction was significantly reduced. M-cholinergic receptor blocking agents atropine, ipratropium, pirenzepine, and 4-DAMP also significantly reduced the melanoidin-provoked contraction. Nonspecific N-cholinergic receptor blockers hexamethonium and decamethonium (1 × 10 mol L¹ each) did not influence the melanoidin-induced mechanical reaction. The melanoidins did not affect the strength of contractions evoked by adrenaline and dopamine (1 × 10 mol L¹ each).
The results obtained support the assumption that melanoidin-evoked contraction is a result of activation of muscarinic-type cholinergic receptors.