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Antitrypanosomal activity of aloin and its derivatives against Trypanosoma congolense field isolate

Identifier

020989

Type of Spiritual Experience

Background

A description of the experience

BMC Vet Res. 2014 Mar 10;10:61. doi: 10.1186/1746-6148-10-61.

Antitrypanosomal activity of aloin and its derivatives against Trypanosoma congolense field isolate.

Tewabe Y, Bisrat D, Terefe G, Asres K1.

  • 1Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia. kaleab.asres@aau.edu.et.

Abstract

BACKGROUND:

There is an urgent need for the development of new, cheap, safe and highly effective drugs against African trypanosomiasis that affects both man and livestock in sub-Saharan Africa including Ethiopia. In the present study the exudate of Aloe gilbertii, an endemic Aloe species of Ethiopia, aloin, aloe-emodin and rhein were tested for their in vitro and in vivo antitrypanosomal activities against Trypanosoma congolense field isolate. Aloin was prepared from the leaf exudate of A. gilbertii by acid catalyzed hydrolysis. Aloe-emodin was obtained by oxidative hydrolysis of aloin, while rhein was subsequently derived from aloe-emodin by oxidation. In vitro trypanocidal activity tests were conducted on parasites obtained from infected mice, while mice infected with T. congolense were used to evaluate in vivo antitrypanosomal activity of the test substances.

RESULTS:

Results of the study showed that all the test substances arrested parasites motility at effective concentration of 4.0 mg/ml within an incubation period ranging from 15 to 40 min. Moreover, the same concentration of the test substances caused loss of infectivity of the parasites to mice during 30 days observation period. Among the tested substances, rhein showed superior activity with minimum inhibitory concentration (MIC) of 0.4 mg/ml. No adverse reactions were observed when the test substances were administered at a dose of 2000 mg/kg. Rhein at doses of 200 and 400 mg/kg, and the exudate, aloin and aloe-emodin at a dose of 400 mg/kg reduced the level of parasitaemia significantly (P < 0.05) and improved anaemia.

CONCLUSION:

The results obtained in this investigation indicate that aloin and its derivatives particularly rhein have the potential to be used for the development of safe and cost effective antitrypanosomal drugs that can be useful in the continuing fight against African trypanosomiasis.

PMID:

24612613

The source of the experience

PubMed

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