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Antimicrobial activity of essential oil and aqueous and ethanol extracts of Teucrium polium L. subsp. gabesianum (L.H.) from Tunisia



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Physiol Mol Biol Plants. 2017 Jul;23(3):723-729. doi: 10.1007/s12298-017-0444-9. Epub 2017 May 12.
Antimicrobial activity of essential oil and aqueous and ethanol extracts of Teucrium polium L. subsp. gabesianum (L.H.) from Tunisia.
Ben Othman M1, Bel Hadj Salah-Fatnassi K2, Ncibi S3, Elaissi A4, Zourgui L1.

  • 1Unit of Researche of Macromolecular Biochemistry and Genetic, Faculty of Sciences of Gafsa, 2112 Zarroug City, Gafsa Tunisia.
  • 2Laboratory of Transmissible Diseases and Biologically Active Substances, Faculty of Pharmacy, 5000 Monastir, Tunisia.
  • 3Jazan University, Faculty of Science Jazan, Jazan, Saudi Arabia.
  • 4Laboratory of Pharmacognosy and Phytotherapy, Faculty of Pharmacy, Monastir, 5000 Tunisia.


The antimicrobial effects of essential oil, ethanol and aqueous extracts of Teucrium polium L. were investigated against 13 microorganisms. Extracts and essential oil were obtained from maceration, decoction and hydrodistillation respectively. Samples were tested for their antimicrobial activity using the disk diffusion, the agar dilution and the agar incorporation method. Essential oil was analysed using GC/MS, results showed that β-pinene (35.97%) and α-pinene (13.32%) were the main components. Furthermore, essential oil exhibited the highest antimicrobial activity, it was most effective against

  • Proteus mirabilis,
  • Staphylococcus aureus and
  • Citrobacter freundei

where inhibition zone ranged between 15 and 25 mm, and with the microbial inhibitory concentration (MIC) values of 0.078-0.156 mg/ml.

The oil and ethanol extract showed the best antifungal activity against

  • Microsporum canis,
  • Scopulariopsis brevicaulis, and
  • Trichophyton rubrum

with the inhibition percentage (I%) ranging from 18.94 to 100%. However, none of the samples exhibited antifungal activity against Aspergillus fumigatus.

In this study, the obtained results showed significant effects of essential oils and ethanol extracts of T. polium which may used as a substitute to the synthetic drugs against certain microbial diseases.
Antibacterial activity; Antifungal activity; Essential oil; Teucrium polium L. subsp. gabesianum

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