Int Immunopharmacol. 2015 Feb;24(2):325-34. doi: 10.1016/j.intimp.2014.12.026. Epub 2014 Dec 31.

Total glucosides of paeony inhibit the inflammatory responses of mice with allergic contact dermatitis by restoring the balanced secretion of pro-/anti-inflammatory cytokines.

Wang C1, Yuan J1, Wu HX1, Chang Y1, Wang QT1, Wu YJ1, Zhou P1, Yang XD1, Yu J1, Wei W2.

• 1Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine of Education Ministry of China, Mei shan Road, Hefei 230032, China.
• 2Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine of Education Ministry of China, Mei shan Road, Hefei 230032, China. Electronic address: wwei@ahmu.edu.cn.

Abstract

The present study aimed to investigate the regulation exerted by the total glucosides of paeony (TGP) on the production of interleukin-2 (IL-2), IL-4, IL-10 and IL-17 in the serum and lymphocytes of mice with allergic contact dermatitis (ACD). ACD in mice was induced by the repeated application of 2,4-dinitrochlorobenzene (DNCB) to their skins.

The mice were orally administered TGP (35, 70, and 140mg/kg/d) and prednisone (Pre, 5mg/kg/d) from day 1 to day 7 after immunization.

The inflammatory responses were evaluated by ear swelling and histological examination.

Thymocyte proliferation was assayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H tetrazolium bromide assay.

The cytokine production in the serum and lymphocytes supernatant was measured by enzyme-linked immunosorbent assay.

The results indicated that the topical application of DNCB to the skin provoked obvious inflammatory responses. The oral administration of TGP (70 and 140mg/kg/d) and Pre (5mg/kg/d) significantly inhibited skin inflammation, decreased the thymus and spleen indices, and inhibited thymocyte proliferation in mice treated with DNCB. Further study indicated that TGP increased IL-4 and IL-10 production but decreased the production of IL-2 and IL-17 in the serum and lymphocyte supernatant. The correlation analysis suggested significantly positive correlations between IL-2 and IL-17 production and the severity of skin inflammation, whereas negative correlations were obtained for IL-4 and IL-10 production and skin inflammation.

In summary, these results suggest that the therapeutic effects of TGP on ACD may result from its regulation of the imbalanced secretion of IL-2/IL-4 and IL-10/IL-17.

Copyright © 2014 Elsevier B.V. All rights reserved.

KEYWORDS:

Allergic contact dermatitis; Interleukin-10; Interleukin-17; Interleukin-2; Interleukin-4; Total glucosides of paeony

PMID:

25556068