Avicenna J Phytomed. 2015 May-Jun;5(3):231-7.

Protective effect of Hibiscus sabdariffa against serum/glucose deprivation-induced PC12 cells injury.

Bakhtiari E1, Hosseini A2, Mousavi SH3.

• 1Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ; Neurocognitive Research center , School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
• 2Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
• 3Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ; Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

OBJECTIVES:

Findings natural products with antioxidant and antiapoptotic properties has been one of the interesting challenges in the search for the treatment of neurodegenerative diseases including ischemic stroke. Serum/glucose deprivation (SGD) has been used as a model for the understanding of the molecular mechanisms of neuronal damage during ischemia in vitro and for the expansion of neuroprotective drugs against ischemia-induced brain injury. Recent studies showed that Hibiscus sabdariffa exert pharmacological actions such as potent antioxidant. Therefore, in this study we investigated the protective effect of extract of H. sabdariffa against SGD-induced PC12 cells injury.

MATERIALS AND METHODS:

Cells were pretreated with different concentrations of H. sabdariffa extract (HSE) for 2 hr, and then exposed to SGD condition for 6, 12 and 18 hr.

RESULTS:

SGD caused a major reduction in cell viability after 6, 12, and 18 hr as compared with control cells (p< 0.001). Pretreatment with HSE (30-500