Acaricidal activity of oregano oil and its major component, carvacrol, thymol and p-cymene against Psoroptes cuniculi in vitro and in vivo
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Vet Parasitol. 2016 Aug 15;226:93-6. doi: 10.1016/j.vetpar.2016.07.001. Epub 2016 Jul 2. Acaricidal activity of oregano oil and its major component, carvacrol, thymol and p-cymene against Psoroptes cuniculi in vitro and in vivo. Shang X1, Wang Y1, Zhou X1, Guo X1, Dong S1, Wang D1, Zhang J1, Pan H1, Zhang Y2, Miao X3. 1Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Science, Lanzhou 730050, PR China.
Oregano oil possesses marked antioxidant and antimicrobial activity and is widely applied in animal husbandry.
In the present study, we aimed to investigate the acaricidal activities of oregano oil and its major component, carvacrol, thymol and p-cymene against Psoroptes cuniculi in vitro and in vivo.
The results revealed that oregano oil exhibited significant acaricidal effects against P. cuniculi that were dose- and time-dependent response. In in vitro test, concentrations of 0.05% and 0.02% (v/v) killed all of the mites within 1h and 6h, respectively. Moreover, 0.1mg/ml (w/v) carvacrol, 0.2mg/ml (w/v) thymol and 1% p-cymene (v/v) also possessed marked acaricidal activities, and compared with the control group, elicited mean mortalities of 84.00%, 96.00% and 66% at 24h, respectively. The median lethal times (LT50) against P. cuniculi of the concentrations of 0.02%, 0.01% and 0.005% (v/v) of oregano oil, thymol, carvacrol and p-cymene were 2.171h, 11.396h, 26.102h, and 4.424h, 8.957h and 15.201h, respectively.
Meanwhile, twenty naturally infested rabbits were used to four homogeneity groups: negative control (without treatment), positive control (treated with ivermectin), group treated with 1% of oregano oil and other group with 5% of oregano oil. All the treatments were topically.
After the treatment of 1% and 5% oregano oil, the P. cuniculi were completely eliminated in the rabbits, and at the end of the test (day 20), the rabbits of all treatment groups exhibited favourable mental and physical statuses. These results indicated that oregano oil could be widely applied as a potential acaricidal agent in the treatment of animal acariasis in the future.