Mol Med Rep. 2015 Jun;11(6):4611-6. doi: 10.3892/mmr.2015.3310. Epub 2015 Feb 6.

Safflower polysaccharide inhibits the proliferation and metastasis of MCF-7 breast cancer cell.

Luo Z1, Zeng H2, Ye Y3, Liu L2, Li S2, Zhang J1, Luo R1.

• 1Department of Oncology, Cancer Center of Southern Medical University, Guangzhou, Guandong 510315, P.R. China.
• 2Department of Chemotherapy, Ganzhou Cancer Hospital, Ganzhou, Jiangxi 341000, P.R. China.
• 3Department of Breast Surgery, Ganzhou Cancer Hospital, Ganzhou, Jiangxi 341000, P.R. China.

Abstract

Breast cancer accounts for 22.9% of all types of cancer in females worldwide. Safflower polysaccharide (SPS) is an active fraction purified from safflower petals (Carthamus tinctorius L). The present study investigated the effects of safflower polysaccharide on the proliferation and metastasis of breast cancer cells. Cell viability was analyzed using an MTT assay following treatment of the MCF‑7 cells with increasing concentrations of SPS. The results demonstrated that the SPS compound significantly inhibited the proliferation of the MCF‑7 human breast cancer cell line and these inhibitory effects increased in a dose‑ and time‑dependent manner. The half maximal inhibitory concentration (IC50) value of SPS on breast cancer cells, following treatment for 72 h, was detected using an MTT assay and was calculated as 0.12 mg/ml. The apoptotic rate was detected using flow cytometry in the MCF‑7 human breast cancer cell line and the results revealed that SPS induced cell apoptosis. The apoptotic rate of the MCF‑7 cells treated with SPS was significantly higher compared with that of the untreated cells and increased in a dose‑dependent manner. The expression of B‑cell lymphoma 2 (Bcl‑2) was downregulated and the expression of Bcl‑2‑associated X protein was upregulated in the MCF‑7 cells treated with SPS in a time‑dependent manner. Additionally, the expression of matrix metalloproteinase‑9 was significantly reduced and the expression of tissue inhibitor of metalloproteinase‑1 was increased in the MCF‑7 human breast cancer cell treated with SPS. These results demonstrated that SPS inhibited the metastasis of MCF‑7 breast cancer cells and understanding the underlying mechanisms may provide novel strategies in breast cancer therapy.

PMID:  25673029