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Role of Carum copticum seeds in modulating chromium-induced toxicity on human bronchial epithelial cells and human peripheral blood lymphocytes

Identifier

027886

Type of Spiritual Experience

Background

A description of the experience

Exp Toxicol Pathol. 2012 Nov;64(7-8):889-97. doi: 10.1016/j.etp.2011.03.012. Epub 2011 Apr 22.
Role of Carum copticum seeds in modulating chromium-induced toxicity on human bronchial epithelial cells and human peripheral blood lymphocytes.
Deb DD1, Parimala G, Devi SS, Chakrabarti T.
Author information
 
Abstract
Carum copticum seeds are well known for ailment of various diseases since ancient times. The present study pertains to investigate modulatory effects of methanolic extract of C. copticum seeds (MCE) against hexavalent chromium induced cytotoxicity, genotoxicity, apoptosis and oxidative stress on human bronchial epithelial cells (BEAS-2B) and isolated human peripheral blood lymphocyte (PBL) in vitro. Treatment of BEAS-2B and PBL with MCE prior to potassium dichromate (K(2)Cr(2)O(7)) treatment exhibited an increase in cell viability and decrease of DNA damage as compared to K(2)Cr(2)O(7) treatment alone, as evaluated by WST-8 and Comet assay respectively. Further, MCE administration 1h prior to graded doses of K(2)Cr(2)O(7) significantly decreased reactive oxygen species (ROS) level, increased the mitochondrial membrane potential, reduced apoptosis and caspase 3 activity. MCE also ameliorated K(2)Cr(2)O(7) induced decrease in superoxide dismutase (SOD), glutathione peroxidase (GPx) antioxidant enzyme levels in BEAS-2B and PBL cells accompanied by reduction in lipid peroxides with maximum effect at 50 μg/ml. Thus, this study provides strong evidence to support the beneficial effect of MCE in preventing Cr(VI) induced toxicity in BEAS-2B and PBL cells.
PMID:
21515038

The source of the experience

PubMed

Concepts, symbols and science items

Concepts

Symbols

Science Items

Activities and commonsteps

Activities

Overloads

Chromium imbalance

Suppressions

Ajwan

Commonsteps

References