Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "MANOSROI III"
Type of Spiritual Experience
A description of the experience
Pharm Biol. 2015 Jul;53(7):1075-81. doi: 10.3109/13880209.2014.959613. Epub 2015 Jan 23.
Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "MANOSROI III".
Manosroi A1, Akazawa H, Pattamapun K, Kitdamrongtham W, Akihisa T, Manosroi W, Manosroi J.
- 1Faculty of Pharmacy, Chiang Mai University , Chiang Mai , Thailand .
Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including oral and cervical cancers.
To investigate anti-proliferative activity on human cervical (HeLa) and oral (KB) cancer cell lines of medicinal plants selected from Thai/Lanna medicinal plant recipe database "MANOSROI III".
MATERIALS AND METHODS:
Twenty-three methanolic plant crude extracts were tested for phytochemicals and anti-proliferative activity on HeLa and KB cell lines for 24 h by the sulforhodamine B (SRB) assay at the doses of 1 × 10(1)-1 × 10(-6 )mg/ml. The nine extracts with the concentrations giving 50% growth inhibition (GI50) lower than 100 µg/ml were further semi-purified by liquid/liquid partition in order to evaluate and enhance the anti-proliferative potency.
All extracts contained steroids/triterpenoids, but not xanthones. The methanolic extracts of Gloriosa superba L. (Colchinaceae) root and Albizia chinensis (Osbeck) Merr. (Leguminosae-Mimosoideae) wood gave the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.91 (6.0- and 0.31-fold of cisplatin and doxorubicin) and 0.16 µg/ml (28.78- and 82.29-fold of cisplatin and doxorubicin), respectively. Hexane and methanol-water fractions of G. superba exhibited the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.15 (37- and 1.9-fold of cisplatin and doxorubicin) and 0.058 µg/ml (77.45- and 221.46-fold of cisplatin and doxorubicin), respectively.
DISCUSSION AND CONCLUSION:
This study has demonstrated the potential of plants selected from MANOSROI III database especially G. superba and A. chinensis for further development as anti-oral and cervical cancer agents.
Anti-proliferative effects; cervical cancer; oral cancer; thai medicinal plants; “MANOSROI III” database
The source of the experiencePubMed
Concepts, symbols and science items
Activities and commonsteps
Mouth and tooth disease
Reproductive system disease