Metagenomic testing of mouth biome for pathogens causing SLE [systemic lupus erythematosus]
Type of Spiritual Experience
A description of the experience
Microbiome. 2017 Mar 20;5(1):34. doi: 10.1186/s40168-017-0252-z.
Subgingival microbiota dysbiosis in systemic lupus erythematosus: association with periodontal status.
Corrêa JD1, Calderaro DC2, Ferreira GA2, Mendonça SM1, Fernandes GR3, Xiao E4, Teixeira AL2, Leys EJ5, Graves DT4, Silva TA6,7.
Periodontitis results from the interaction between a subgingival biofilm and host immune response. Changes in biofilm composition are thought to disrupt homeostasis between the host and subgingival bacteria resulting in periodontal damage. Chronic systemic inflammatory disorders have been shown to affect the subgingival microbiota and clinical periodontal status. However, this relationship has not been examined in subjects with systemic lupus erythematosus (SLE). The objective of our study was to investigate the influence of SLE on the subgingival microbiota and its connection with periodontal disease and SLE activity.
We evaluated 52 patients with SLE compared to 52 subjects without SLE (control group). Subjects were classified as without periodontitis and with periodontitis. Oral microbiota composition was assessed by amplifying the V4 region of 16S rRNA gene from subgingival dental plaque DNA extracts. These amplicons were examined by Illumina MiSeq sequencing.
SLE patients exhibited higher prevalence of periodontitis which occurred at a younger age compared to subjects of the control group. More severe forms of periodontitis were found in SLE subjects that had higher bacterial loads and decreased microbial diversity. Bacterial species frequently detected in periodontal disease were observed in higher proportions in SLE patients, even in periodontal healthy sites such as Fretibacterium, Prevotella nigrescens, and Selenomonas. Changes in the oral microbiota were linked to increased local inflammation, as demonstrated by higher concentrations of IL-6, IL-17, and IL-33 in SLE patients with periodontitis.
SLE is associated with differences in the composition of the microbiota, independently of periodontal status.
Cytokine; Illumina sequencing; Lupus; Oral microbiota; Periodontitis; Subgingival dental plaque
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