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Fitoterapia. 2015 Sep;105:169-76. doi: 10.1016/j.fitote.2015.07.003. Epub 2015 Jul 7.  Chemo-protective and regenerative effects of diarylheptanoids from the bark of black alder (Alnus glutinosa) in human normal keratinocytes. Dinić J1, Ranđelović T2, Stanković T2, Dragoj M2, Isaković A3, Novaković M4, Pešić M5.   Institute for Biological Research, Department of Neurobiology, University of Belgrade, Bulevar Despota Stefana 142, Belgrade, Serbia. Electronic address: jelena.dinic@ibiss.bg.ac.rs.

2Institute for Biological Research, Department of Neurobiology, University of Belgrade, Bulevar Despota Stefana 142, Belgrade, Serbia.

3Faculty of Medicine, University of Belgrade, Doktora Subotića 8, Belgrade, Serbia.

4Institute for Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia.

5Institute for Biological Research, Department of Neurobiology, University of Belgrade, Bulevar Despota Stefana 142, Belgrade, Serbia. Electronic address: camala@ibiss.bg.ac.rs.

Abstract

Medicinal plants are recognized from ancient times as a source of diverse therapeutic agents and many of them are used as dietary supplements. Comprehensive approaches are needed that would identify bioactive components with evident activity against specific indications and provide a better link between science (ethno-botany, chemistry, biology and pharmacology) and market.

Recently, the bark of black alder (Alnus glutinosa) appeared at market in the form of food supplement for treatment of different skin conditions.   This study aimed to evaluate protective effects of two diarylheptanoids isolated from the bark of black alder:

• platyphylloside, 5(S)-1,7-di(4-hydroxyphenyl)-3-heptanone-5-O-β-D-glucopyranoside (1) and its newly discovered analog
• 5(S)-1,7-di(4-hydroxyphenyl)-5-O-β-D-[6-(E-p-coumaroylglucopyranosyl)]heptane-3-one (2)

 towards doxorubicin damaging activity. To that end, we employed HaCaT cells, non-cancerous human keratinocytes commonly used for skin regenerative studies.

Diarylheptanoids significantly antagonized the effects of doxorubicin by lowering the sensitivity of HaCaT cells to this drug. Compound 2 prevented doxorubicin-induced cell death by activating autophagy. Both 1 and 2 protected HaCaT cells against doxorubicin-induced DNA damage. They significantly promoted migration and affected F-actin distribution.

These results indicate that chemo-protective effects of diarylheptanoids may occur at multiple subcellular levels. Therefore, diarylheptanoids 1 and 2 could be considered as protective agents for non-cancerous dividing cells during chemotherapy.

Copyright © 2015 Elsevier B.V. All rights reserved.

KEYWORDS:

Cell motility; Chemoprotection; Curcumin; DNA damage; Diarylheptanoid; Doxorubicin

PMID:  26162555