Antioxidant phenolic compounds from Alchornea floribunda leaves
Type of Spiritual Experience
A description of the experience
Antioxidant phenolic compounds from Alchornea floribunda leaves Article in Planta Medica 79(13) · August 2013 DOI: 10.1055/s-0033-1352198
Blessing OGECHUKWU Umeokoli 20.81Nnamdi Azikiwe University, Awka O Obasi
Daniel Lotanna Ajaghaku 16.45Enugu State University of Science and Technology BF Okoye
Alchornea floribunda leaves have been previously shown to posses potent anti-inflammatory compounds1 – 3. Further chemical investigation of a flavonoid sub-fraction of the methanol leaf extract of Alchornea floribunda led to the isolation of three flavans (-) catechin (+) catechin (-) epicatechin and a flavanone (2R, 3R) dihydroquercitin.
The structures of these compounds were elucidated by 1 D and 2 D NMR spectroscopy and Liquid Chromatography- Electrospray Ionization Mass Spectrometry (LC-ESIMS). All the isolated compounds were assessed for their anti-oxidant potential in vitro using DPPH free radical scavenging and Ferric reducing ability⁴. In the DPPH model, (-) epicatechin, (+) epicatechin and (2R, 3R) dihydroquercitin showed high free radical scavenging activity with IC50 values of 19, 40 and 46 µg/mL respectively with the activity of (-) epicathechin comparable to that of the standard, chlorogenic acid (IC50 = 22 µg/mL). (-) catechin showed only mild activity with IC50 value of 88 µg/mL. In the Ferric reducing test, (-) catechin, (-) epicatechin and (+) epicatechin exhibited IC50 values of 46, 68 and 88 22 µg/mL respectively with the activity of (-) epicathechin higher than that of the standar, ascobic acid (IC50 = 66 µg/mL). (2R, 3R) dihydroquercitin showed poor Ferric reducing activity (IC50 > 100 µg/mL).
The observed antioxidant activities of the isolated flavans may explain the efficacy of the extract of Alchornea floribunda leaves in the ethnomedicinal management of diseases associated with oxidative stress.
The source of the experiencePubMed
Concepts, symbols and science items
Activities and commonsteps
SuppressionsAlchornea floribunda (Niando, Iporuru, Iporoni and Macochihua)
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