Anticancer properties of low molecular weight oat beta-glucan - An in vitro study
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Int J Biol Macromol. 2015 Sep;80:23-8. doi: 10.1016/j.ijbiomac.2015.05.035. Epub 2015 Jun 16.
Anticancer properties of low molecular weight oat beta-glucan - An in vitro study.
Choromanska A1, Kulbacka J2, Rembialkowska N2, Pilat J2, Oledzki R3, Harasym J3, Saczko J2.
- 1Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland. Electronic address: firstname.lastname@example.org.
- 2Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
- 3Department of Food Biotechnology, Wroclaw University of Economics, Komandorska 118-120, 53-345 Wroclaw, Poland.
Anticancer properties of 1-3, 1-4 oat beta glucan are under intensive investigation now.
Antitumor characteristic of fungi and yeast beta-glucans have been widely recognized, but those polysaccharides are mostly insoluble which creates several problems especially in topical formulation.
Also high molecular weight oat beta-glucans reveal high viscosity which restricts its application.
According to those problems in the current study the antitumor activities of low molecular weight beta-glucan derived from oats were investigated in cancer cells: Me45, A431 and normal HaCaT and murine macrophages P388/D1.
The low molecular weight beta-glucan from oat significantly decreased cancer cells viability, while for the normal cells it was non-toxic. It was observed that with the increasing incubation time and the beta-glucan concentration the cancer cells viability significantly decreased. Furthermore for the normal cells the low molecular weight beta-glucan from oat was non-toxic.
Immunocytochemical ABC analysis showed that beta-glucan induced strong expression of caspase-12 in both cancer cell lines, while in HaCaT cells ABC reaction was significantly lower and in P388/D1 cell line ABC reaction was negative.
Our preliminary studies show strong anti-tumor properties of new low molecular weight beta-glucan from oat and at the same time no toxicity for normal cells.
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Melanoma; Oat beta-glucan; Skin cancer