Observations placeholder
Does the sampling locality influence on the antifungal activity of the flavonoids of Marrubium vulgare against Aspergillus niger and Candida albicans
Identifier
023031
Type of Spiritual Experience
Background
A description of the experience
J Mycol Med. 2016 Sep;26(3):201-11. doi: 10.1016/j.mycmed.2016.02.019. Epub 2016 Mar 17. [Does the sampling locality influence on the antifungal activity of the flavonoids of Marrubium vulgare against Aspergillus niger and Candida albicans?]. [Article in French] Bouterfas K1, Mehdadi Z2, Aouad L3, Elaoufi MM4, Khaled MB4, Latreche A2, Benchiha W2.
OBJECTIVE: The study was undertaken to determine the effect of the sampling locality on the antifungal activity of the flavonoids extracted from the leaves of Marrubium vulgare L. against two fungal strains; Aspergillus niger ATCC 16404 and Candida albicans ATCC 10231.
MATERIALS AND METHODS:
The leaves were collected from three different sampling localities belonging northwest Algeria: Tessala mount, M'sila forest and Ain Skhouna. The flavonoid extraction was carried out by using organic solvents with increasing polarity. A phytochemical screening was performed by staining test tubes. The inhibition diameters were measured by solid medium diffusion method. The minimum inhibitory concentrations were determined by dilution method on solid medium.
RESULTS:
The antifungal activity varied significantly (P<0.001) according to the sampling locality of the leaves, the flavonoid extract and its concentration, and the strain fungal type. The inhibition diameters varied between 8.16 and recorded 37.5mm even recording a total inhibition of fungal growth and often exceed those induced marketed antifungals (Amphotericin, Fluconazole, Terbinafine and econazole nitrate). The minimum inhibitory concentrations (MICs) obtained range between 6.25 and 100μg/mL; experiencing strong antifungal inhibition. The phytochemical screening revealed the existence of certain flavonoids classes such as flavans and flavanols which may be responsible of this remarkable antifungal power.
CONCLUSION:
The sampling locality of Marrubium vulgare leaves influenced on the antifungal activity of flavonoids. These have proven very good fungistatic and worth valuing in pharmacology.
Copyright © 2016 Elsevier Masson SAS. All rights reserved.
KEYWORDS:
Activité antifongique; Antifungal activity; Flavonoids; Flavonoïdes; Localité d’échantillonnage; Marrubium vulgare; Sampling locality
PMID: 26994761